Outline
Introduction (Figure 26.1)
DNA as the Template for RNA Synthesis
The Predicted Existence of Messenger RNA (Figure 26.2)
T2 Bacteriophage and the Demonstration of Messenger RNA (Figure 26.3)
RNA Dynamics in Uninfected Cells
Enzymology of RNA Synthesis: RNA Polymerase (Item #1, p. 960, #2)
Biological Role of RNA Polymerase (Figure 26.4)
Mechanism of Transcription
Initiation of Transcription: Interactions with Promoters (Figure 26.6)
Initiation and Elongation: Incorporation of Ribonucleotides (Figure 26.8)
Punctuation of Transcription: Promoter Recognition (Figure 26.11, Figure 26.12, Figure 26.14)
Punctuation of Transcription: Termination (Figure 26.15)
Factor-Dependent Termination (Figure 26.16)
Regulation of Transcription
The Lactose Operon: Early Evidence for Transcriptional Control of Gene Expression
Regulation in the Lactose Operon (Figure 26.17, Figure 26.18)
Isolation and Properties of Repressor
The Repressor Binding Site (Figure 26.10, Figure 26.19)
Regulation of the lac Operon by Glucose: A Positive Control System (Figure 26.21)
The CRP-DNA Complex
Bacteriophage
: Multiple Operators, Dual Repressors, and Models for DNA Binding Specificity (Figure 26.23)
Genes and Mutations in the
The
Early Genes in Phage
Structure of Cro and cI Repressors and Related DNA-Binding Proteins (Figure 26.28, Figure 26.29, Figure 26.30, Figure 26.31)
The SOS Regulon: Activation of Multiple Operons by a Common Set of Environmental Signals (Figure 26.32)
Biosynthetic Operons: Ligand-Activated Repressors and Attenuation (Figure 21.14, Figure 26.33, Figure 26.35, Figure 26.36, Figure 26.15)
Other Forms of Regulation
The Galactose Operon (Figure 26.38)
The Arabinose Operon (Figure 26.39)
Control by Antisense RNA (Figure 26.40)
Posttranscriptional Processing
Posttranscriptional Processing in the Synthesis of rRNA and tRNA
rRNA Processing (Figure 26.41)
tRNA Processing (Figure 26.42)