Trans-2-enoyl-S-CoA is an intermediate in fatty acid oxidation. It is formed in the following reaction,

Fatty acyl-S-CoA + E-FAD <=> Trans-2-enoyl-S-CoA + E-FADH2 (catalyzed by Fatty Acyl-CoA Dehydrogenase)(Figure 18.16). The FAD and FADH2 in the reaction are enzyme-bound. Electrons from FADH2 are donated to Coenzyme Q in the electron transport system (Figure 18.17).

and is converted to L-3-hydroxyacyl-S-CoA in the next reaction:

Trans-2-Enoyl-S-CoA + H2O <=> L-3-Hydroxyacyl-S-CoA (catalyzed by Enoyl-CoA Hydratase)(Figure 18.16)

-Oxidation of Saturated Fatty Acid,

, Oxidation of Odd-Numbered Fatty Acids, Peroxisomal -Oxidation, Fatty Acids