Enoyl-CoA hydratase catalyzes addition of water to trans-2-enoyl-S-CoA in the process of fatty acid oxidation (Figure 18.16). The product of this reaction is the stereospecific L isomer of 3-hydroxylacyl-CoA.

Trans-2-Enoyl-S-CoA + H2O <=> L-3-Hydroxyacyl-S-CoA

-Oxidation of Saturated Fatty Acid,

, Oxidation of Odd-Numbered Fatty Acids, Peroxisomal -Oxidation, Fatty Acids, Figure 18.16