L-3-Hydroxyacyl-S-CoA is an intermediate in fatty acid oxidation (Figure 18.16) that participates in the reactions below:

1. Trans-2-Enoyl-S-CoA + H2O <=> L-3-Hydroxyacyl-S-CoA (catalyzed by Enoyl-CoA Hydratase)

2. L-3-Hydroxyacyl-S-CoA + NAD⁺ <=> 3-Ketoacyl-S-CoA + NADH + H⁺  (catalyzed by 3-Hydroxyacyl-CoA Dehydrogenase)

-Oxidation of Saturated Fatty Acid,

, Oxidation of Odd-Numbered Fatty Acids, Peroxisomal -Oxidation, Fatty Acids