The enzyme enolase catalyzes the interconversion of 2-phosphoglycerate and phosphoenolpyruvate.
2-Phosphoglycerate <=> Phosphoenolpyruvate + H2O (
= 1.7 kJ/mol)
The reaction, which involves removal of a water molecule from 2-phosphoglycerate to form phosphoenolpyruvate, occurs in both glycolysis and gluconeogenesis .
Previous step of glycolysis; Next step of glycolysis