3-Phosphoglycerate is an intermediate in the glycolysis, gluconeogenesis, and Calvin cycle pathways and in metabolism of serine, cysteine, and glycine. 3-Phosphoglycerate is acted on by the glycolytic enzymes phosphoglycerate kinase and phosphoglycerate mutase and the important photosynthesis enzyme, ribulose-1,5-bisphosphate carboxylase (rubisco).