2-Phosphoglycerate is an intermediate in the glycolysis and gluconeogenesis pathways.

2-Phosphoglycerate is acted on by the glycolytic enzymes phosphoglycerate mutase and enolase. When fluoride ion is added to yeast extracts undergoing fermentation, 2-phosphoglycerate is one of the products that accumulates. This is due to inhibition of the enolase enzyme by the fluoride ion.