A Lineweaver Burk plot (Figure 11.16)is a way of plotting kinetic enzyme data so as to yield a straight line for reactions obeying Michaelis-Menten kinetics. This is done by plotting 1/V versus 1/[S], where V is the reaction velocity and [S] is the substrate concentraton. A Lineweaver Burk plot yields values for -1/KM (x-intercept) and 1/Vmax (y-intercept).

A disadvantage of the Lineweaver-Burk plot is the long extrapolation necessary to determine KM. One way around this problem is to graph V versus V/[S] (called an Eadie-Hofstee plot). The result, shown in Figure 11.17 also yields a straight line for reactions obeying Michaelis-Menten kinetics. Here, the slope of the line is equal to -KM and Vmax is obtained form the x-intercept.