Eadie Hofstee Plot

The Eadie-Hofstee plot (Figure 11.17) is a way of plotting kinetic enzyme data so as to yield a straight line for reactions obeying Michaelis-Menten kinetics. This is done by plotting reaction velocity (V) versus velocity/substrate concentration (V/[S]). The slope of the line is equal to -KM and the x-intercept is Vmax.

An advantage of an Eadie-Hofstee plot over a Lineweaver Burk plot (Figure 11.16) (which plots 1/V versus 1/S) is that the Eadie-Hofstee plot does not require a long extrapolation to calculate KM.