Biochemistry Laboratory: Modern Theory and Techniques
Rodney F. Boyer, Hope College

ISBN-10: 0805346139
ISBN-13: 9780805346138

Publisher: Prentice Hall
Copyright: 2006
Format: Paper; 400 pp
Published: 06/27/2005

Suggested retail price: $65.80
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Many biochemistry lab instructors are now opting to either design their own experiments or select them from major educational journals. Biochemistry Laboratory: Modern Theory and Techniques addresses this issue by providing a flexible alternative without experimental protocols.  Instead of requiring instructors to use specific experiments, the book focuses on detailed descriptions of modern techniques in experimental biochemistry and discusses the theory behind such techniques in detail.  The extensive range of techniques includes internet databases, chromatography, electrophoresis, spectroscopy, measurements of ligand-binding interactions, and recombinant DNA techniques such as molecular cloning and PCR.

 

  • Includes a substantially expanded and modernized first part drawn from Boyer’s successful Modern Experimental Biochemistry text, providing in-depth theoretical discussion organized around important techniques. 
  • Provides an extensive range of techniques including internet databases, chromatography, electrophoresis, spectroscopy, measurements of ligand-binding interactions, and recombinant DNA techniques such as molecular cloning and PCR.
  • Unique organization offers flexibility to accommodate various requirements of the course, and allows students to reference detailed theory sections for clarification during labs.  Also includes information on a variety of teaching methods for the lab.

 

Part I: Theory and Experimental Techniques

  1. Introduction to the Biochemistry Laboratory
  2. The Computer as a Tool in Biochemistry and Molecular Biology
  3. General Laboratory Procedures
  4. Centrifugation Techniques in Biochemistry
  5. Purification and Identification of Biomolecules by Chromatography
  6. Characterization of Proteins and Nucleic Acids by Electrophoresis
  7. Spectroscopic Analysis of Biomolecules
  8. Biomolecular Interactions: Ligand Binding and Enzyme Reactions
  9. Molecular Biology I: Structures and Analysis of Nucleic Acids
  10. Molecular Biology II: Recombinant DNA. Molecular Cloning, and Enzymology
  11. Protein Production, Purification, and Characterization

Part II: Teaching the Biochemistry/Molecular Biology Lab

    1. A Brief History
    2. A Variety of Teaching Methods
    3. Essential BMB Concepts and Skills for Student Learning
    4. Experiments in Biochemistry and Molecular Biology

Further Reading

Appendices.

Index.

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