Enzymatic reactions are key concepts for biology students to understand. Various properties of enzymatic reactions are observed by photometrically measuring the initial rate of synthesis of a product. The student can vary temperature, pH, initial substrate concentration, enzyme concentration, and the concentration of several different inhibitors. A sophisticated graphing function is included so that students can easily plot their data on a normal graph or on reciprocal plots. pH and temperature optimums, Michaelis-Menton constants such as Km, Ki and Vmax, and the different classes of inhibitors are some of the enzymatic principles that can be investigated with this lab.

The purpose of this laboratory is to:

• Understand properties of enzymatic reactions.

• Simulate the photometric measurement of enzyme-catalyzed reactions.

• Investigate pH and temperature optimums, Michaelis-Menten enzyme kinetics, and different classes of inhibitors.